pET vector

pET vector is an expression vector driven by T7 promoter. On the lineup of BDL, there are 3 types of vectors: high copy, medium copy, and vectors whose expression can be controlled by LacI. And each drug resistance marker can be selected from 2 types: ampicillin and kanamycin. Furthermore, depending on the insertion site, it is possible to express His-Tag fusion proteins or untagged proteins.

The T7 expression system is a powerful expression system. A pET expression vector with a T7 promoter and E. coli containing a T7 RNA polymerase gene such as BL21 (DE3) are used. The expression of the T7 RNA polymerase gene under the control of the lac UV5 promoter in the BL21(DE3) genome is induced by the addition of isopropyl-1-thio-β-D-galactopyranoside (IPTG). The expressed T7 RNA polymerase recognizes the T7 promoter of the pET vector and strongly expresses the target protein downstream of the T7 promoter.

-6 vectors have the same multi-cloning site. Therefore, subcloning into other vectors in the series can be easily performed.
-Due to the small size of the vector, highly efficient transformation can be expected even with large insertions.
-Proteins with a His tag fused to the N-terminus can be expressed. Additionally, by inserting the target gene into the NdeI site, untagged proteins can be expressed.

If the effect of the expressed protein on the host E. coli is unclear, or if you are unsure about vector selection, please try pETBA.
If you want to suppress expression during non-induction, pETIA is recommended.
When the expressed protein does not have much effect on the host E. coli, using pETUA has the advantage of making it easier to perform miniprep and sequencing because the plasmid has a large copy number.
pETUK, pETBK, and pETIK have similar features to pETUA, pETBA, and pETIA, respectively. Kanamycin-resistant vectors are useful for subcloning from ampicillin-resistant vectors.