A Novel Running Buffer that enable Gradient-gel-like Electrophoresis
AllView PAGE Buffer
AllView PAGE Buffer (20×) is a novel running buffer for SDS-PAGE electrophoresis. This buffer enables protein separation with wide range of molecular weight withount gradient gel. By simply replacing the usual running buffer with AllView PAGE Buffer, you can achieve gradient-gel-like electrophoresis using the basic Laemmli gel (Tris-HCl).
Highlights
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No need for expensive gradient gels
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Electrophoresis completed in 15 minutes (mini gel size, 250V)
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Conventional analysis such as CBB staining, silver staining, western blotting is possible.
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Higher blotting efficiency is achieved via 6% AA gels.
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20 x solution
＜Video＞
Change the electrophoresis buffer to AllView PAGE Buffer, and get wide separation and 15 minutes electrophoresis!
AllView PAGE Buffer is suitable for Laemmli gels.
*When using precast gel, the applied gel concentration may be different.
Conventional SDS-PAGE buffer vs AllView PAGE Buffer
Using a self-made gel with Tris- HCl buffer (Laemmli method), the conventional SDS-PAGE electrophoresis buffer (left, Tris-Glycine-SDS: Laemmli method) and AllView PAGE Buffer (right) were compared.
At 6% gel concentration, conventional buffers could not separate proteins below 45 kDa, but AllView PAGE Buffer allowed a wide range of separations, from 8 to 230 kDa, as in a gradient gel.
Sample: DynaMarker Protein MultiColor Stable II
*The gel self-made by Laemmli method is recommended. When using precast gel, the applied gel concentration may be different.
Citations
Guo Y. et al., elife, 13:e95856 (2024).
Nemoto H. et al., PLoS One, 17(12):e0278613 (2022).
EN
BDL is a member of the Funakoshi Group.
